限饲对湖羊子宫RGMb基因及BMP系统成员表达的影响

doi:10.11843/j.issn.0366-6964.2017.05.010

Abstract

Abstract:

The study aimed to explore the effects of food restriction on the expression of RGMb and BMP system members in the uterus of Hu ewes. Firstly, qPCR were applied to investigate the RGMb expression profile in different tissues of 3-month-old Hu ewes, IHC were used to detect the expression of RGMb in uterus. Then Hu ewes on 35 days of pregnancy (d35) were divided into 2 groups: ewes in the control (C) group were fed with 100% NRC and ewes in the restriction (R) group were fed with 50% NRC. On 110 days of pregnancy, uterine tissues (n=8) were collected to evaluate the expression of BMP2, BMP4, BMPR1A, BMPR1B, BMPR2 and RGMb by qPCR and WB. qPCR results showed that RGMb mRNA were detected in 14 tissues of 3-month-old Hu ewes, and the relative expression was greater than 0.5 in uterus. IHC staining showed that RGMb protein were mainly expressed in the endometrial luminal and glandular epithelial cells of Hu ewes. The uterine expression of RGMb protein in the R group was significantly lower (P<0.05) than that in the C group, while there was no significant differences in RGMb mRNA abundance(P>0.05). The expression of BMP4 and BMP2 mRNAs in the R group were higher (P<0.05) than those in the C group. However, the expression of BMP receptors(BMPR1A, BMPR1B and BMPR2) significantly decreased in the R group(P<0.05). The results indicate that food restriction affect the expression of BMP system members, including the RGMb, suggesting RGMb accompanied with BMP system may be involved in the regulation of uterine hemeostasis, which will set up experimental evidences for the further study on the regulation mechanism of BMP system in the uterus.

CLC Number:

S826
S815.4

KUANG Mei-qian, JIN Peng-jin, WANG Ruo-cheng, SUN Ling-wei, WANG Feng, LI Peng, CHENG Zhi-jun, MAO Da-gan. Effect of Food Restriction on the Expression of RGMb and BMP System Members in the Uterus of Hu Ewes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(5): 863-870.

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References

[1] ZHANG H, BRADLEY A. Mice deficient for BMP2 are nonviable and have defects in amnion/chorion and cardiac development[J]. Development, 1996, 122(10): 2977-2986.
[2] TSUJI K, BANDYOPADHYAY A, HARFE B D, et al. BMP2 activity, although dispensable for bone formation, is required for the initiation of fracture healing[J]. Nat Genet, 2006, 38(12): 1424-1429.
[3] LI Y, WEI Q W, FENG J G, et al. Expression of bone morphogenetic protein 2, 4, and related components of the BMP signaling pathway in the mouse uterus during the estrous cycle[J]. J Zhejiang Univ Sci B, 2014, 15(7): 601-610.
[4] VISSER J A, OLASO R, VERHOEF-POST M, et al. The serine/threonine transmembrane receptor ALK2 mediates Müllerian inhibiting substance signaling[J]. Mol Endocrinol, 2001, 15(6): 936-945.
[5] GOUÉDARD L, CHEN Y G, THEVENET L, et al. Engagement of bone morphogenetic protein type IB receptor and Smad1 signaling by anti-Müllerian hormone and its type II receptor[J]. J Biol Chem, 2000, 275(36): 27973-27978.
[6] YI S E, LAPOLT P S, YOON B S, et al. The type I BMP receptor BMPRIB is essential for female reproductive function[J]. Proc Natl Acad Sci U S A, 2001, 98(14): 7994-7999.
[7] 柳江枫, 杨宝学. RGMb/DRAGON介导的信号通路及其研究进展[J]. 神经药理学报, 2014, 4(2): 46-54.
LIU J F, YANG B X. RGMb/DRAGON mediated signaling pathways[J]. Acta Neuropharmacologica, 2014, 4(2): 46-54. (in Chinese)
[8] YURA S, ITOH H, SAGAWA N, et al. Role of premature leptin surge in obesity resulting from intrauterine undernutrition[J]. Cell Metab, 2005, 1(6): 371-378.
[9] 孟晨玲. RGMb基因在大鼠发情周期子宫和卵巢及湖羊组织中的表达研究[D]. 南京: 南京农业大学, 2014.
MENG C L. Expression patterns of RGMb in rat uterus and ovary during the estrous cycle and its expression in Hu sheep[D]. Nanjing: Nanjing Agricultural University, 2014. (in Chinese)
[10] MENG C L, LIU W, HUANG H, et al. Repulsive guidance molecule b (RGMb) is dispensable for normal gonadal function in mice[J]. Biol Reprod, 2016, 94(4): 78.
[11] 石国庆, 茆达干, 程瑞禾, 等. 湖羊和新疆细毛羊妊娠早期内分泌比较[J]. 南京农业大学学报, 2008, 31(1): 146-148.
SHI G Q, MAO D G, CHENG R H, et al. Comparison of endocrinology during early pregnancy of Hu sheep and Xinjiang fine wool sheep[J]. Journal of Nanjing Agricultural University, 2008, 31(1): 146-148. (in Chinese)
[12] 管峰, 艾君涛, 刘守仁, 等. BMPR-IB和BMP15基因作为湖羊多胎性候选基因的研究[J]. 家畜生态学报, 2005, 26(3): 9-12.
GUAN F, AI J T, LIU S R, et al. Study of BMPR-IB and BMP15 as candidate genes for prolificacy in Hu sheep[J]. Acta Ecologiae Animalis Domastici, 2005, 26(3): 9-12. (in Chinese)
[13] 茆达干, 石国庆, 张红琳, 等. 湖羊早期妊娠的子宫内环境研究[J]. 畜牧兽医学报, 2010, 41(9): 1203-1207.
MAO D G, SHI G Q, ZHANG H L, et al. Study on intrauterine environment during early pregnancy of Hu sheep[J]. Acta Veterinaria et Zootechnica Sinica, 2010, 41(9): 1203-1207. (in Chinese)
[14] ZHANG H, SUN L W, WANG Z Y, et al. Dietary N-carbamylglutamate and rumen-protected-arginine supplementation ameliorate fetal growth restriction in undernourished ewes[J]. J Anim Sci, 2016, 94(5): 2072-2085.
[15] LANGLEY-EVANS S C, DANIEL Z C, WELLS C A, et al. Protein restriction in the pregnant mouse modifies fetal growth and pulmonary development: role of fetal exposure to β-hydroxybutyrate[J]. Exp Physiol, 2011, 96(2): 203-215.
[16] WU G Y, BAZER F W, SATTERFIELDET M C, et al. Impacts of arginine nutrition on embryonic and fetal development in mammals[J]. Amino Acids, 2013, 45(2): 241-256.
[17] MENG C L, GUO N N, WEI Q W, et al. Expression of repulsive guidance molecule b (RGMb) in the uterus and ovary during the estrous cycle in rats[J]. Acta Histochem, 2014, 116(8): 1231-1236.
[18] GUO N N, MENG C L, BAI W J, et al. Prostaglandin F_2α induces expression of activating transcription factor 3 (ATF3) and activates MAPK signaling in the rat corpus luteum[J]. Acta Histochem, 2015, 117(2): 211-218.
[19] WEI Q W, SHI F X. Cleavage of poly (ADP-ribose) polymerase-1 is involved in the process of porcine ovarian follicular atresia[J]. Anim Reprod Sci, 2013, 138(3-4): 282-291.
[20] SAMAD T A, REBBAPRAGADA A, BELL E, et al. DRAGON, a bone morphogenetic protein co-receptor[J]. J Biol Chem, 2005, 280(14): 14122-14129.
[21] XIA Y, SIDIS Y, MUKHERJEE A, et al. Localization and action of Dragon (repulsive guidance molecule b), a novel bone morphogenetic protein coreceptor, throughout the reproductive axis[J]. Endocrinology, 2005, 146(8): 3614-3621.
[22] 孟晨玲, 王亚磊, 郭南南, 等. 湖羊RGMb基因的克隆、序列分析及表达[J]. 畜牧与兽医, 2014, 46(6): 59-62.
MENG C L, WANG Y L, GUO N N, et al. Cloning, sequence analysis and expression of RGMb gene from Hu sheep[J]. Animal Husbandry & Veterinary Medicine, 2014, 46(6): 59-62. (in Chinese)
[23] MA C H, BRENNER G J, OMURA T, et al. The BMP coreceptor RGMb promotes while the endogenous BMP antagonist noggin reduces neurite outgrowth and peripheral nerve regeneration by modulating BMP signaling[J]. J Neurosci, 2011, 31(50): 18391-18400.
[24] XIAO Y P, YU S H, ZHU B G, et al. RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance[J]. J Exp Med, 2014, 211(5): 943-959.
[25] ERICKSON G F, FUQUA L, SHIMASAKI S. Analysis of spatial and temporal expression patterns of bone morphogenetic protein family members in the rat uterus over the estrous cycle[J]. Endocrinology, 2004, 182(2): 203-217.
[26] 胡兰. 动物生物化学[M]. 北京: 中国农业大学出版社, 2007.
HU L. Animal biochemistry[M]. Beijing: China Agricultural University Press, 2007. (in Chinese)
[27] GU L B, ZHU N X, ZHANG H Y, et al. Regulation of XIAP translation and induction by MDM2 following irradiation[J]. Cancer Cell, 2009, 15(5): 363-375.
[28] LIU G, LIN H, ZHANG X, et al. Expression of Smad2 and Smad4 in mouse uterus during the oestrous cycle and early pregnancy[J]. Placenta, 2004, 25(6): 530-537.
[29] PARIA B C, DAS S K, ANDREWS G K, et al. Expression of the epidermal growth factor receptor gene is regulated in mouse blastocysts during delayed implantation[J]. Proc Natl Acad Sci U S A, 1993, 90(1): 55-59.
[30] SHIMASAKI S, MOORE R K, OTSUKA F, et al. The bone morphogenetic protein system in mammalian reproduction[J]. Endocr Rev, 2004, 25(1): 72-101.
[31] TANWAR P S, MCFARLANE J R. Dynamic expression of bone morphogenetic protein 4 in reproductive organs of female mice[J]. Reproduction, 2011, 142(4): 573-579.
[32] MONSIVAIS D, CLEMENTI C, PENG J, et al. Uterine ALK3 is essential during the window of implantation[J]. Proc Natl Acad Sci U S A, 2016, 113(3): E387-E395.
[33] LEE M J, YANG C W, JIN D C, et al. Bone morphogenetic protein-7 inhibits constitutive and interleukin-1β-induced monocyte chemoattractant protein-1 expression in human mesangial cells: role for JNK/AP-1 pathway[J]. J Immunol, 2003, 170(5): 2557-2563.
[34] MIYAZAKI Y, OSHIMA K, FOGO A, et al. Evidence that bone morphogenetic protein 4 has multiple biological functions during kidney and urinary tract development[J]. Kidney Int, 2003, 63(3): 835-844.
[35] WILLETTE R N, GU J L, LYSKO P G, et al. BMP-2 gene expression and effects on human vascular smooth muscle cells[J]. J Vasc Res, 1999, 36(2): 120-125.

Effect of Food Restriction on the Expression of RGMb and BMP System Members in the Uterus of Hu Ewes

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